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This volume compiles a broad range of step-by-step protocols complementary to the ones published in the first edition of this book to study various aspects of mitochondrial structure and function in different model organisms both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Mitochondria: Practical Protocols Second Edition aims to be useful for beginners as well as for experienced researchers in the field.

Binding : Gebundene Ausgabe, Edition : 1, Label : Jessica Kingsley Publishers, Publisher : Jessica Kingsley Publishers, medium : Gebundene Ausgabe, numberOfPages : 336, publicationDate : 2018-04-19, releaseDate : 2018-04-19, authors : Ray Griffiths, ISBN : 1848193327

This book shows how the mitochondrial genome is transmitted segregated and inherited. Opens by describing mtDNA mutations and deletions and showing how these impact the offspring's well-being covers mtDNA transmission and discusses transmittal and adaptation.

This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR) mitochondrial membrane potential and measuring mitochondrial Ca2+ handling and ROS emission. Written in the format of the highly successful Methods in Molecular Biology series each chapter includes an introduction to the topic lists necessary materials and reagents includes tips on troubleshooting and systematic reproducible protocols. Authoritative and cutting-edge Mitochondria: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Brand : HAY HOUSE, Binding : Gebundene Ausgabe, Label : Hay House, Publisher : Hay House, medium : Gebundene Ausgabe, numberOfPages : 360, publicationDate : 2022-05-10, releaseDate : 2022-05-10, authors : Ari Whitten, Leaf, M.S., Alex, ISBN : 1401964966

This volume compiles a broad range of step-by-step protocols complementary to the ones published in the first edition of this book to study various aspects of mitochondrial structure and function in different model organisms both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Mitochondria: Practical Protocols Second Edition aims to be useful for beginners as well as for experienced researchers in the field.

This book is indispensable to researchers in fields as diverse as molecular biology and biophysics. It is an essential text for university libraries covering as it does the important role that mitochondria play in a variety of biochemical spheres.

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology function and features of mitochondria. In the past decade mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology biochemistry physiology dynamics and morphology and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study such as the examination of apoptotic effects fusion and fission of mitochondria and proteome and transcriptome analysis.

Mitochondria are organelles in each cell outside the nucleus and are the energy source of all cells. As such they are crucial to the healthy functioning of cells. Recent research has shown that mitochondrial dysfunction underlies a broad spectrum of disease from maternally inherited genetic disorders to metabolism defects aging stroke and neurodegenerative diseases such as Parkinson's Alzheimer's and Lou Gehrig's disease. This book brings together top researchers whose work in examining the pathophysiologic processes will lead to new strategies for prevention and treatment.

This volume details comprehensive protocols and methodologies to assess mitochondrial bioenergetics and dynamics in different tissues and cells involving health and pathological states. Chapters guide readers through methods for assessment of the energy metabolism including Oxygen Consumption Rate (OCR) mitochondrial membrane potential and measuring mitochondrial Ca2+ handling and ROS emission. Written in the format of the highly successful Methods in Molecular Biology series each chapter includes an introduction to the topic lists necessary materials and reagents includes tips on troubleshooting and systematic reproducible protocols. Authoritative and cutting-edge Mitochondria: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Mitochondria are the product of a long evolutionary history. It is now a well established fact that mitochondria did evolve from free living bacteria being the common ancestor of both eukaryotic mitochondria and ?-proteobacteria. Advances in genome sequencing the establishment of in organello and in vitro assays to name only a few contributed significantly to advances in plant mitochondrial research. Second generation sequencing and the ability to directly sequence and analyse the whole plant transcriptome certainly will help to develop the research on plant mitochondria to another level in the future. In this book the current knowledge about plant mitochondria is presented in a series of detailed chapters which have been organized in five main sections: (i) dynamics genes and genomes; (ii) transcription and RNA processing; (iii) translation and import; (iv) biochemistry regulation and function; and (v) mitochondrial dysfunction and repair. These sections consist of two to five chapters each written by well-known specialists in the field. This book thus provides a comprehensive inside in the field of plant mitochondria for the specialist. The addition of a glossary and text boxes to each chapter provides easy access for readers from other subjects and hopefully will attract young scientist to the fascinating and exiting field of plant mitochondria.

This book is indispensable to researchers in fields as diverse as molecular biology and biophysics. It is an essential text for university libraries covering as it does the important role that mitochondria play in a variety of biochemical spheres.

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology function and features of mitochondria. In the past decade mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology biochemistry physiology dynamics and morphology and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study such as the examination of apoptotic effects fusion and fission of mitochondria and proteome and transcriptome analysis.

This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging replication genome integrity and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods including for mtDNA isolation visualization deep sequencing gene editing and diagnostic aspects of mtDNA disease. Written in the format of the highly successful Methods in Molecular Biology series each chapter includes an introduction to the topic lists necessary materials and methods includes tips on troubleshooting and known pitfalls and step-by-step readily reproducible protocols. Authoritative and cutting-edge Mitochondrial DNA: Methods and Protocols aims to be useful and informative for researchers and clinicians with an interest in mitochondrial DNA.

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today starting from the isolation of mitochondria to detailed analyses of RNA protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA techniques to assess mitochondrial DNA damage visualization of mitochondrial DNA in situ detection of mitochondrial DNA nucleoids within the mitochondria methods for analyzing mitochondrial DNA replication mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number the latest technologies for modifying the mitochondrial genome and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Mitochondrial DNA: Methods and Protocols Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor including cancer and neurodegeneration.

This volume focuses on mitochondrial RNA metabolism emphasizing recent discoveries and technological advances in this fast moving area that increase our understanding of mitochondrial gene function. Topics addressed include the interplay of mitochondria with the nucleus and cytosol structure-function connections and relevance to human disease. Mitochondria are the powerhouses of the cell and a great deal is known about mitochondrial energy metabolism. Less well known is the plethora of amazing mechanisms that have evolved to control expression of mitochondrial genomes. Several RNA processes and machineries in protozoa plants flies and humans are discussed including: transcription and RNA polymerase mechanism; tRNA processing of 5' and 3' ends; mRNA maturation by nucleotide insertion/deletion editing and by RNA splicing; mRNA stability; and RNA import. Specialized factors and ribonucleoproteins (RNPs) examined include pentatricopeptide repeat (PPR) proteins RNase P polymerases helicases nucleases editing and repair enzymes. Remarkable features of these processes and factors are either not found outside mitochondria differ substantially among eukaryotic lineages or are unique in biology.

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today starting from the isolation of mitochondria to detailed analyses of RNA protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.

This special book is conceived to highlight mitochondrial structural and functional integrity and how they are associated with several human diseases such as cardiovascular cancer renal neurological disorder and genetic disorders. The chapters contributed by leading mitochondrial researchers in the handbook will take us through the novel pharmacological strategies via mitochondria to understand their physiological and pathological role as well as present them as therapeutic targets.

Mitochondria have been pivotal in the development of some of the most important ideas in modern biology. Since the discovery that the organelle has its own DNA and specific mutations were found in association with neuromuscular and cardiovascular diseases and with aging an extraordi-nary number of publications have followed and the term mitochondrial medicine was coined. Furthermore our understanding of the multiple roles that mitochondria play in cardiac cell homeostasis opened the door for intensive experimentation to understand the pathogenesis and to find new treatments for cardiovascular diseases. Besides its role in adenosine triphosphate generation mitochondria regu-late a complex network of cellular interactions involving (1) generation and detoxification of reactive oxygen species including superoxide anion hy-drogen peroxide and hydroxyl radical; (2) maintenance of the antioxidant glutathione in a reduced state and adequate level of mitochondrial matrix superoxide dismutase; (3) cytoplasmic calcium homeostasis particularly under conditions of cellular calcium loading; (4) transport of metabolites between cytoplasm and matrix; (5) both programmed (apoptosis) and necrotic cell death; and (6) cell growth and development. It is therefore not surprising that this organelle has come to be the center stage in many current investigations of cardiovascular diseases aging and agi- related disease. Concomitant with these advances an impressive effort is under- way for the development of new tools and methodologies to study mitochondrial structure and function including powerful ways to visualize monitor and alter the organelle function to assess the genetic consequences of these perturbations.